Multiple myeloma panel | FISH

Test DetailsDetects common gene rearrangements associated with MM: D13S319 del13q, FGFR3/IGH t(4;14), CCND1/IGH t(11;14), TP53 17p13, CKS1B/CDKN2C 1p32.3/1q21, IGH/MAF t(14;16)
Relevant condition(s)Multiple myeloma
MethodologyFluorescence in-situ hybridization
PerformedMonday – Saturday
Turnaround3 – 5 days; STAT FISH performed within 24 – 48 hours
Specimen requirementsSuspension FISH on Bone marrow, Bone core, Lymph node or Peripheral Blood ONLY
Sample collectionIn sterile 15 mL sodium heparin (green-top) vacutainer tube collect 1 – 2 mLs bone marrow; 5 – 10 mLs blood; 0.5 – 1.0 cm2 biopsy (bone core; lymph node); invert tube immediately after collection to prevent clots
Minimum sample volume0.5 mLs bone marrow; 2 mLs blood
Storage/Transport ConditionsRoom temperature
Unacceptable ConditionsFrozen specimens, clotted or hemolyzed specimens, inappropriate tube type, paraffin sample; Will not be accepted after three days
Special InstructionsRefrigerate if sample cannot be delivered within 24 hours; Send to Rapid Response or SLCH Central Receiving
CPT Code(s)88291×1, 88237×1, 88271×8, 88275×4