Detection of Acid – fast bacilli

Alpha smooth muscle isoforms, myoepithelium etc.

Acid mucopolysaccharides

Acid and neutral mucins

It is expressed in only few cells in the developing and mature nervous system Ð glial cells, neurons, endothelial cells and pericytes.

Detection of androgen receptors.  Primarily used in carcinomas of the breast and prostate.

Follicular lymphoma, other B and T cell lymphomas, soft tissue tumors

Follicular lymphoma, follicular center B cells, DLBCL subset

Detection of the antigen in postmortem, formalin fixed and paraffin embedded tissue

Fibromatosis, colorectal carcinoma

Demonstration of Bile pigments

Detection of BK (SV40 family) virus in post-transplant kidney

Gastrointestinal stromal tumors, mast cells

Used to aid in the characterization of lymphoma.

Pancreas, GI, ovary, lung and transitional cell carcinoma

Non-mucinous ovarian carcinoma, serous and endometroid adenocarcinomas

Thyroid medullary carcinoma, thyroid C cells

Normal and reactive mesothelium, mesothelioma and distinguishing mesothelioma from adeocarcinoma

Pan – T cell – thymic and peripheral T lymphocytes, mast cells, NK cells, T cell lymphoma subsets

Normal follicular dendritic cells; dendritic cell tumor/sarcomas, B cell subset

B-CLL, Small lymphocytic (B cell) lymphoma

Normal and neoplastic T cells

Mononuclear Hodgkin's cells, Reed Sternberg cells of Hodgkin's lymphoma and anaplastic large cell lymphoma; plasma cells

Endothelium, vascular neoplasms, plasma cells

Myeloid progenitor cells, endothelium, vascular tumors

Neural and neuroendocrine tumor, soft tissue tumor subset, NK cells, plasma and T-cell subsets

Neural and neuroendocrine tumor, follicular center cell lymphoma

Megakaryocytes, platelets

Macrophages

Macrophages

Thymocytes and mature T cells

Pan B cells

Cytotoxic / suppressor T cells

T cells, cortical thymocytes, Ewing's sarcoma / PNET, sex cord stromal tumors

Enteric differentitation, GI tumors, colorectal carcinoma, GI origin of metastatic adenocarcinomas and carcinoids. Mucinous carcinomas of the ovary

Neuroendocrine neoplasms

Acid mucopolysaccharides and sialomucins

Demonstrtion of amyloid in tissues

Staining copper in tissues

Mantle cell lymphoma, breast cancer

Normal and abnormal epithelial cells and to determine lineage of poorly differentiated tumors

Variety of epithelium and epithelial malignancies including adenocarcinoma of colon, stomach, pancreas, biliary tract and breast

GI tumors, transitional cell carcinoma, Merkel cell tumors

Tumors with squamous differentiation, malignant mesothelioma versus adenocarcinoma of lung, myopeithelial cells, benign vs. malignant breast intraductal proliferation

Normal and neoplastic cells of ovary, lung and breast. Often used in conjunction with CK20

Found in most simple epithelium. Adenocarcinomas and most non-keratinizing squamous carcinomas. Will stain Paget cells.

Squamous cell and subsets of adenocarcinoma and transitional cell carcinoma, prostate basal cells, hyperplastic prostate versus prostate cancer

Lymphatic endothelium, lymphangiomas, Kaposi sarcomas and subset of angiosarcomas.

Skeletal and smooth muscle tumors

 Distinguishes between lobular and ductal epithelial proliferations

Stain for elastic fibers, collagen, and cardiac or skeletal muscle

Identification of epithelial membrane antigen in adenocarcinomas of secretory epithelium, malignant mesothelioma, renal cell carcinoma and meningiomas

Staining method for acid fast bacilli

Stain for argentaffin material. For diagnosis of melanomas, carcinoid tumors and pheochromocytoms

Expressed in breast carcinoma and urothelial carcinoma

Erythroid cell precursors, red blood cells and erythroid leukemias

Demonstration of Fungi

Used for the semi-quantitative detection of HER2 antigen in neoplastic breast tissue.

Used as a melanocyte marker to aid in the differential diagnosis of melanocytic verses non-melanocytic tumors.

Used as a melanocyte marker to aid in the differential diagnosis of melanocytic verses non-melanocytic tumors.

To distinguish malignant rhabdoid tumors and other malignant central nervous system tumors

Demonstration of iron/hemosiderin in tissues

Ventana Ultra

Ventana Ultra

Ventana Ultra

Demonstrates glomerular basement membrane

Cell proliferation marker

Granulocytes and histiocytes. Marker for leukemias and histiocytic neoplasms

Breast carcinoma, salivary gland lesion and sweat gland carcinoma

Melanocytic lesions

Identify DNA mismatch repair protein

Mouse monoclonal antibody directed against mismatch repair protein MSH2. This antibody is used in the identification of microsatellite instability.

Identify DNA mismatch repair protein

B cells in germinal centers, plasma cells, activated T cells and related hematolymphoid neoplasmas. Along with CD30 used as a marker for Hodgkin's and reed Sternberg cells

Rhabdomyosarcoma

Mouse monoclonal antibody directed against napsin which is a pepsin like aspartic proteinase. High expression is seen is lung and kidney

Neural, neuroendocrine and endocrine tumors

Seminoma, dysgerminoma, embryonal carcinoma