BCL6 _ 3q27 | FISH
| Test Details | Detects rearrangements of 3q27 |
| Relevant condition(s) | CLL, Lymphoma |
| Methodology | Fluorescence in-situ hybridization |
| Performed | Monday – Friday |
| Turnaround | 3 – 5 days; STAT FISH performed within 24 – 48 hours |
| Specimen requirements | Suspension FISH on bone marrow, bone core, lymph node, peripheral blood, and FFPE |
| Sample collection | Suspension: In sterile 15 mL sodium heparin (green-top) vacutainer tube collect 1 – 2 mLs bone marrow; 5 – 10 mLs blood; 0.5 – 1.0 cm2 biopsy (bone core; lymph node); invert tube immediately after collection to prevent clots; FFPE: Formalin-fixed paraffin embedded tissue samples (blocks or unstained slides) |
| Minimum sample volume | Suspension: 0.5 mLs bone marrow; 2 mLs blood; FFPE: Not applicable as long as tumor cells are present |
| Storage/Transport Conditions | Room temperature |
| Unacceptable Conditions | Suspension: Will not be accepted after three days; FFPE: Tissue samples on un-charged microscope slides, previously stained slides (H&E, IHC, etc.) |
| Special Instructions | Suspension: Refrigerate if sample cannot be delivered within 24 hours; Send to Rapid Response or SLCH Central Receiving |
| CPT Code(s) | Suspension: 88291×1, 88237×1, 88271x 2, 88275×1; FFPE: 88368×2 |