Aneuscreen (prenatal) | FISH

Test DetailsAneuVysion Multicolor DNA Probe Kit detects aneuploidy of chromosomes 13, 18, 21, X and Y
MethodologyFluorescence in-situ hybridization
PerformedMonday – Saturday
Turnaround4 – 5 days; STAT aneuscreens performed within 24 – 48 hours
Specimen requirementsAmniotic fluid (AF), chorionic villi sampling (CVS), percutaneous umbilical blood sampling (PUBS)
Sample collectionAF: Discard first 2mL of amniotic fluid. Collect 15-25mL in a sterile syringe. Use sterile technique to transfer to 15mL sterile screw-top plastic conical tubes for transport. Do not centrifuge. CVS: Collect 15-40mg of budding villi in 50mL screw-top plastic conical tubes in sterile transport media (provided by cytogenetics lab). Do not centrifuge. PUBS: Collect 1-3mL in sterile, 15mL sodium heparin (green-top) Vacutainer tube. Collect under ultrasound guidance. Invert tube immediately upon completion of blood collection to prevent formation of clots.
Minimum sample volume 
Storage/Transport ConditionsRoom temperature
Unacceptable ConditionsWill not be accepted after three days
Special InstructionsRefrigerate if sample cannot be delivered within 24 hours; Send to Rapid Response or SLCH Central Receiving
CPT Code(s)AF: 88291×1, 88235×1, 88271×5, 88275×2; CVS: 88291×1, 88235×1, 88271×5, 88275×2