AML panel | FISH

Test DetailsDetects common gene rearrangements associated with AML: PML/RARA t(15;17), RUNX1/RUNX1T1 t(8;21), CBFB inv(16), KMT2A (MLL) 11q23, CEP8, D7S486 -7/del(7), EGR1 -5/del(5)
Relevant condition(s)AML
MethodologyFluorescence in-situ hybridization
PerformedMonday – Saturday
Turnaround3 – 5 days; STAT FISH performed within 24 – 48 hours
Specimen requirementsSuspension FISH on Bone marrow, Bone core, Lymph node or Peripheral Blood ONLY
Sample collectionIn sterile 15 mL sodium heparin (green-top) vacutainer tube collect 1 – 2 mLs bone marrow; 5 – 10 mLs blood; 0.5 – 1.0 cm2 biopsy (bone core; lymph node); invert tube immediately after collection to prevent clots
Minimum sample volume0.5 mLs bone marrow; 2 mLs blood
Storage/Transport ConditionsRoom temperature
Unacceptable ConditionsFrozen specimens, clotted or hemolyzed specimens, inappropriate tube type, paraffin sample; Will not be accepted after three days
Special InstructionsRefrigerate if sample cannot be delivered within 24 hours; Send to Rapid Response or SLCH Central Receiving
CPT Code(s)88291×1; 88237×1; 88271×13; 88275×8