T-cell ALL panel | FISH

Test DetailsDetects common gene rearrangements associated with T-Cell ALL; TCR(TRA/D) 14q11, TCRB(TRB) 7q34, KMT2A (MLL) 11q23, CDKN2A 9p21
MethodologyFluorescence in-situ hybridization
PerformedMonday – Saturday
Turnaround3 – 5 days; STAT FISH performed within 24 – 48 hours
Specimen requirementsSuspension FISH on Bone marrow, Bone core, Lymph node or Peripheral Blood ONLY
Sample collectionIn sterile 15 mL sodium heparin (green-top) vacutainer tube collect 1 – 2 mLs bone marrow; 5 – 10 mLs blood; 0.5 – 1.0 cm2 biopsy (bone core; lymph node); invert tube immediately after collection to prevent clots
Minimum sample volume0.5 mLs bone marrow; 2 mLs blood
Storage/Transport ConditionsRoom temperature
Unacceptable ConditionsFrozen specimens, clotted or hemolyzed specimens, inappropriate tube type, paraffin sample; Will not be accepted after three days
Special InstructionsRefrigerate if sample cannot be delivered within 24 hours;
Send to Clinical Support Office (509 S. Euclid Ave., Room 4711 between 8 a.m. and 5 p.m.)
After hours, please send to IOH Core Lab (425 S. Euclid, Room 4701)
CPT Code(s)88291×1; 88237×1; 88271×8; 88275×4