B-cell ALL panel | FISH

Test DetailsDetects common gene rearrangements associated with B-cell ALL: ETV6/RUNX1 t(12;21), BCR/ABL t(9;22), KMT2A (MLL) 11q23, CEP4, 10, 17 (aneuploidies), TCF3 19p13, CDKN2A del(9)(p21), IGH 14q32, CRLF2 Xp/Yp, ABL1 9q34, ABL2 1q25, PDGFRB 5q32
MethodologyFluorescence in-situ hybridization
PerformedMonday – Saturday
Turnaround3 – 5 days; STAT FISH performed within 24 – 48 hours
Specimen RequirementsSuspension FISH on Bone marrow, Bone core, Lymph node or Peripheral Blood ONLY
Sample CollectionIn sterile 15 mL sodium heparin (green-top) vacutainer tube collect 1 – 2 mLs bone marrow; 5 – 10 mLs blood; 0.5 – 1.0 cm2 biopsy (bone core; lymph node); invert tube immediately after collection to prevent clots
Minimum Sample Volume0.5 mLs bone marrow; 2 mLs blood
Storage/Transport ConditionsRoom temperature
Unacceptable ConditionsFrozen specimens, clotted or hemolyzed specimens, inappropriate tube type, paraffin sample; Will not be accepted after three days
Special InstructionsRefrigerate if sample cannot be delivered within 24 hours;
Send to Clinical Support Office (509 S. Euclid Ave., Room 4711 between 8 a.m. and 5 p.m.)
After hours, please send to IOH Core Lab (425 S. Euclid, Room 4701)
CPT Code(s)88291×1, 88237×1, 88271×24, 88275×11