AML panel | FISH
| Test Details | Detects common gene rearrangements associated with AML: PML/RARA t(15;17), RUNX1/RUNX1T1 t(8;21), CBFB inv(16), KMT2A (MLL) 11q23, CEP8, D7S486 -7/del(7), EGR1 -5/del(5) |
| Relevant condition(s) | AML |
| Methodology | Fluorescence in-situ hybridization |
| Performed | Monday – Saturday |
| Turnaround | 3 – 5 days; STAT FISH performed within 24 – 48 hours |
| Specimen requirements | Suspension FISH on Bone marrow, Bone core, Lymph node or Peripheral Blood ONLY |
| Sample collection | In sterile 15 mL sodium heparin (green-top) vacutainer tube collect 1 – 2 mLs bone marrow; 5 – 10 mLs blood; 0.5 – 1.0 cm2 biopsy (bone core; lymph node); invert tube immediately after collection to prevent clots |
| Minimum sample volume | 0.5 mLs bone marrow; 2 mLs blood |
| Storage/Transport Conditions | Room temperature |
| Unacceptable Conditions | Frozen specimens, clotted or hemolyzed specimens, inappropriate tube type, paraffin sample; Will not be accepted after three days |
| Special Instructions | Refrigerate if sample cannot be delivered within 24 hours; Send to Rapid Response or SLCH Central Receiving |
| CPT Code(s) | 88291×1; 88237×1; 88271×13; 88275×8 |