Specimen handling depends on the type of testing and specimen. Please refer to the links below for more detailed information.
Each specimen must be accompanied by a completed requisition signed by an authorized provider that provides:
- Patient’s name, date of birth, gender, address, and insurance information
- Specimen type, time/date of specimen collection, and test desired
- Clinical indications, history, and therapy
- Referring physician’s name (not resident’s), phone/fax/pager numbers, and address
Improper specimen collection and an incomplete requisition form could cause delays in processing or unsuccessful analyses
Specimen collection instructions
Specific collection instructions are provided to clients, either on the requisition form or as separate collection instructions.
Anatomic pathology & stains
Specimen types accepted include formalin-fixed and paraffin embedded tissue and/or unstained slides (5 micron thick sections). For Ventana IHC, sections should be on Plus(+) charged slides.
Renal pathology
Specimen types accepted include native and transplant kidney biopsies. Please refer to the collection instructions below for more detailed information.
Dermatopathology
Please visit the Dermatopathology website for more information.
Tissue should be excised in 1mm x 1mm or less dimension and fixed in modified Karnovsky’s fixative in a 30:1 fixative to tissue ratio.
Specimen types accepted include formalin-fixed and paraffin embedded tissue and/or unstained slides (5 micron thick sections). For Ventana IHC, sections should be on Plus(+) charged slides.
Cytogenetics
Amniotic Fluide (Amniocentesis)
| Notes | Note the gestational age in weeks; Uniquely identify twins. |
| Collection | Discard first 2mL of amniotic fluid. Collect 15-25mL in a sterile syringe. |
| Processing | Use sterile technique to transfer to 15mL sterile screw-top plastic conical tubes for transport. Do not centrifuge. |
| Shipping | Maintain at room temperature or refrigerate if sample cannot be delivered within 24 hours. Specimen must be received within three days of collection. Send to North Campus Lab or SLCH Central Receiving for delivery to Lab ASAP. |
Chorionic Villi Sampling (CVS)
| Notes | Note the gestational age in weeks; Uniquely identify twins. |
| Collection | Collect 15-40mg of budding villi in 50mL screw-top plastic conical tubes in sterile transport media (provided by cytogenetics lab) |
| Processing | Do not centrifuge. |
| Shipping | Maintain at room temperature or refrigerate if sample cannot be delivered within 24 hours. Specimen must be received within three days of collection. Send to North Campus Lab or SLCH Central Receiving for delivery to Lab ASAP. |
Percutaneous Umbilical Blood Sampling (PUBS)
| Notes | Cordocentesis; periumbilical blood sampling; or fetal blood. |
| Collection | Collect 1-3mL in sterile, 15mL sodium heparin (green-top) Vacutainer tube. Collect under ultrasound guidance. |
| Processing | Invert tube immediately upon completion of blood collection to prevent formation of clots. |
| Shipping | Maintain at room temperature. Send to North Campus Lab or SLCH Central Receiving for delivery to Lab ASAP. |
NOTE: Prefer dual specimens (villi and fetal tissues); must specify type of specimen submitted.
Skin
| Notes | Uniquely identify twins |
| Collection | Collect 3-4mm² skin biopsy (about the size of a pencil eraser) and place in 50ml tube with transport media (provided by the cytogenetics lab). |
| Shipping | Maintain at room temperature or refrigerate if sample cannot be delivered within 24 hours. Specimen must be received within three days of collection. Send to North Campus Lab or SLCH Central Receiving for delivery to Lab ASAP. |
Villi
| Notes | Villi are specimen of choice, but amniotic fluid is acceptable; Uniquely identify twins. |
| Collection | Collect 1 cm² placenta by sterile procedure, and place in 50ml tube with transport media (provided by the cytogenetics lab). |
| Shipping | Maintain at room temperature or refrigerate if sample cannot be delivered within 24 hours. Specimen must be received within three days of collection. Send to North Campus Lab or SLCH Central Receiving for delivery to Lab ASAP. |
Peripheral Blood
| Notes | For CMA studies both sodium heparin (green-top) AND EDTA (purple-top) tubes are required. |
| Collection | Infants, 1-2mL; Children, 3-5mL; Adults, 5-10mL; Collected into a sterile sodium heparin (green-top) Vacutainer tube. |
| Processing | Invert tube immediately upon completion of blood collection to prevent formation of clots. |
| Shipping | Maintain at room temperature or refrigerate if sample cannot be delivered within 24 hours. Samples should be no more than 48 hours old. Send to North Campus Lab or SLCH Central Receiving for delivery to Lab ASAP. |
Skin
| Collection | Collect 3-4mm² skin biopsy (about the size of a pencil eraser) and place in 50ml tube with transport media (provided by the cytogenetics lab). |
| Shipping | Maintain at room temperature or refrigerate if sample cannot be delivered within 24 hours. Send to North Campus Lab or SLCH Central Receiving for delivery to Lab ASAP. |
Hematologic Specimens
| Notes | Spicule-rich bone marrow aspirate; peripheral blood; bone core biopsy; lymph node; or effusion. (Bone marrow is specimen of choice for leukemia. Peripheral blood could be the choice for CLL). |
| Collection | Collect 1-2 mL bone marrow; 5-10 mL blood; 0.5-1 cm2 biopsy (bone core; lymph node). Collect bone marrow or blood in a sodium heparin (green-top) Vacutainer. |
| Processing | Invert tube immediately upon completion of blood collection to prevent formation of clots. For bone marrow it is important that a first draw, spicule-rich sample be collected. |
| Shipping | Maintain at room temperature or refrigerate overnight if necessary. Send to North Campus Lab or SLCH Central Receiving for delivery to Lab ASAP. |
Solid Tumor, Neoplastic Tissue
| Notes | Inform the lab prior to surgery or needle aspiration to facilitate optimal specimen collections and culture set up. |
| Collection | Collect 3-4 mm2 biopsy (about the size of a pencil eraser) in a sterile, screw-top container filled with transport media (provided by cytogenetics lab). |
| Processing | Ensure the container is tightly sealed to prevent leakage. |
| Shipping | Maintain at room temperature or refrigerate if sample cannot be delivered within 24 hours. Send to North Campus Lab or SLCH Central Receiving for delivery to Lab ASAP. |
Paraffin Embedded Tissue (for FFPE FISH)
| Notes | Solid tumors, biopsies, etc. |
| Collection | Tissue fixed in 10% neutral buffered formalin. Preferred fixation duration of 6-48 hours |
| Processing | Place pieces of 4-5 micron thick fixed tissue sections on positively charged slides. Deliver 4 unstained slides per test ordered plus 1 H&E slide marked with region(s) of interest per case/biopsy. |
| Shipping | Maintain at room temperature. Send directly to lab or to BJC IOH Histology laboratory. |
Next-generation sequencing
Specimen types accepted include excisional biopsies, core needle biopsies, cell blocks, leukemic blood or bone marrow aspirate.
Acceptable materials for submission include tumor-containing formalin fixed paraffin embedded (FFPE) blocks, unstained slides from tumor-containing FFPE block, or blood/bone marrow in a lavender-top EDTA tube.
Tissue fixation protocols must be compatible with molecular testing; EDTA decalcification is acceptable, acid decalcification is not.
**Primary specimen type required: tissue from the affected area. Please call the laboratory for further information. In addition, 2-5 mL of peripheral blood in a lavender-top EDTA tube is requested to allow for comparative study.
Acceptable materials for submission include disease involved tissue in the form of a formalin fixed paraffin embedded (FFPE) tissue block, fresh tissue in transport or tissue culture media or buccal swab.
Tissue fixation protocols must be compatible with molecular testing; EDTA decalcification is acceptable, acid decalcification is not.
Specimen types accepted include 2-5 mL peripheral blood in a lavender-top EDTA tube.