p-alpha-Synuclein | IHC
| Test Details | Detection of the antigen in postmortem, formalin-fixed and paraffin embedded brain tissue |
| Components | Clone pSyn#64 performed as an LDT on Ventana Ultra platform |
| Methodology | Immunohistochemistry |
| Performed | Monday – Friday |
| Turnaround | 2 – 3 business days |
| Specimen requirements | FFPE postmortem brain tissue Minimum specimen requirement: 2 slides. If requesting interpretation, please submit an H&E slide. |
| Sample collection | Testing currently limited to WU autopsy cases |
| Unacceptable Conditions | Melted block; brain tissue pre-treated with formic acid |
| CPT Code(s) | NA |
| Miscellaneous | Immunohistochemical tests for alpha-synuclein (clone pSyn#64) were developed by AMP Core Labs in collaboration with the Division of Neuropathology, WU Department of Pathology and Immunology. The tests using DAB or AEC detection were validated for use on formalin-fixed postmortem brain tissues; potential effects of formic acid treatment have not been evaluated. The tests are performed using a polymer-based detection system and instrumentation by Ventana Medical Systems. Additional details regarding the procedures are available on request. Results must be interpreted in conjunction with available clinical and pathologic information. |