B-cell ALL panel | FISH
| Test Details | Detects common gene rearrangements associated with B-cell ALL: ETV6/RUNX1 t(12;21), BCR/ABL t(9;22), KMT2A (MLL) 11q23, CEP4, 10, 17, TCF3 t(1;19)/t(17:19), CDKN2A del(9)(p21), IGH 14q32 |
| Relevant condition(s) | Bcell ALL |
| Methodology | Fluorescence in-situ hybridization |
| Performed | Monday – Saturday |
| Turnaround | 3 – 5 days; STAT FISH performed within 24 – 48 hours |
| Specimen requirements | Suspension FISH on Bone marrow, Bone core, Lymph node or Peripheral Blood ONLY |
| Sample collection | In sterile 15 mL sodium heparin (green-top) vacutainer tube collect 1 – 2 mLs bone marrow; 5 – 10 mLs blood; 0.5 – 1.0 cm2 biopsy (bone core; lymph node); invert tube immediately after collection to prevent clots |
| Minimum sample volume | 0.5 mLs bone marrow; 2 mLs blood |
| Storage/Transport Conditions | Room temperature |
| Unacceptable Conditions | Frozen specimens, clotted or hemolyzed specimens, inappropriate tube type, paraffin sample; Will not be accepted after three days |
| Special Instructions | Refrigerate if sample cannot be delivered within 24 hours; Send to Rapid Response or SLCH Central Receiving |
| CPT Code(s) | 88291×1, 88237×1, 88271×17, 88275×7 |